Hnub no, lub koob yees duab Goryaev yog ib qho ntawm cov khoom siv tshaj plaws hauv kev kuaj mob. Nrog nws cov kev pab, koj tuaj yeem suav cov lej ntawm qee cov qe ntshav. Raws li koj paub, kev kuaj ntshav yog qhov tseem ceeb heev rau kev kuaj mob thiab kho yuav luag txhua yam kab mob.
Goryaev's Chamber yog dab tsi?
Cov khoom siv paub zoo thiab muaj txiaj ntsig zoo li no tau tsim thiab tsim los ntawm tus kws kho mob Lavxias NK Goryaev. Goryaev lub koob yees duab, qhov tseeb, yog ib lub iav zawv zawg nrog ib daim phiaj microscopic siv rau nws.
Lub koob yees duab no siv rau dab tsi? Ua ntej ntawm tag nrho cov, nws yog siv los txiav txim tus naj npawb ntawm cov qe ntshav, tshwj xeeb tshaj yog leukocytes thiab erythrocytes. Los ntawm txoj kev, nrog nws cov kev pab koj tuaj yeem txiav txim siab qhov loj ntawm lub tshuab ntsuas siv. Cov mis rau qhov no yog raws li nram no:
Kg=(m2 – m1)/ aN
Nyob hauv qhov no:
- Kg yog qhov loj ntawm lub tshuab tsom;
- m2 - txoj haujlwm ntawm ciam teb sab xis ntawm Goryaev lub koob yees duab ntawm tes;
- m1 - sab laug ciam teb;
- a - qhov loj ntawm ibhlwb uas yog tus qauv thiab yog 0.05mm;
- N yog pes tsawg lub hlwb ntawm ntsuas lub koob yees duab ciam teb.
Chamber structure
Goryaev lub koob yees duab tsis muaj dab tsi ntau tshaj li lub iav swb (tab sis ntau tuab dua li niaj zaus), muab faib ua peb ntu los ntawm kev sib hloov grooves. Qhov nruab nrab ntawm lub khob muaj ib daim phiaj tshwj xeeb rau kev suav. Qhov kawg ntawm lub chamber pab zom cov npog npog - yog li, lub kaw kaw yog tsim nyob rau hauv qhov chaw nrog capillary qhov chaw ntawm ob sab, los ntawm qhov uas nws tau ntim nrog kua.
Raws li daim phiaj, Goryaev lub xovtooj tau muab faib ua 225 squares loj sib npaug - lawv tau teem rau kaum tsib kab. 25 squares loj ntxiv tau muab faib ua me me, 16 txhua. Qhov ntev ntawm txhua sab ntawm qhov me me square yog 0.05mm.
Npaj suav cov khaub ncaws
Tau kawg, qhov no, cov txheej txheem ntawm kev tshawb fawb hauv chav kuaj yog qhov tseem ceeb heev. Tau kawg, txhua qhov chaw ntawm Goryaev chamber yuav tsum huv si thiab qhuav. Tom qab ntawd, cov npog npog yog rubbed kom pom cov yam ntxwv iridescent rings. Nws yog ib qho tseem ceeb heev uas tsis muaj huab cua npuas nyob rau hauv lub chamber, vim qhov no tej zaum yuav distort cov txiaj ntsig ntawm kev tshuaj ntsuam.
Ib txwm, txhawm rau suav cov lej tsim, cov tshuaj sib txawv yog siv rau txhua hom ntshav. Piv txwv li, 0.9% sodium chloride tov yog siv los suav cov qe ntshav liab. Hauv vitroKoj yuav tsum sib tov 8 ml ntawm ntsev tov thiab 0.02 ml ntawm cov ntshav. Yog li, tus kws pab kuaj ntshav dilutes cov ntshav 400 zaug. Qee zaum kev yug me nyuam tuaj yeem loj.
Yuav kom suav cov leukocytes, koj yuav tsum sib tov 0.4 ml ntawm acetic acid (noj 3% lossis 5% kua) thiab 0.02 ml ntawm cov ntshav.
Tom qab cov khoom sib xyaw ua ke hauv lub raj ntsuas, siv lub raj xa dej tshwj xeeb los sau cov khoom sib xyaw me me thiab ua tib zoo sau cov suav suav (feem ntau ib lossis ob tee yuav txaus).
Yuav ua li cas xam cov qe ntshav?
Erythrocyte suav nyob rau hauv Goryaev lub chamber yog nqa tawm nyob rau hauv tsib squares loj, uas yog sib npaug zos rau yim caum me me. Diagonal squares raug xaiv kom tsis txhob muaj qhov yuam kev vim qhov tsis sib xws ntawm cov ntshav kuaj. Kev saib xyuas tshwj xeeb yog them rau cov hlwb uas nyob ntawm cov npoo - ntawm no lawv suav cov erythrocytes ntawm sab laug thiab sab sauv phab ntsa, tab sis tsis txhob suav rau cov uas nyob rau ntawm kab qis thiab sab xis.
Yuav kom txiav txim seb cov qe ntshav liab nyob rau hauv ib milliliter ntawm cov ntshav, cov qe ntshav nyob rau hauv tsib squares loj yog muab 20,000 (thaum diluted 400 zaug).
Leukocytes hauv Goryaev lub chamber suav txawv. Ntawm no koj yuav tsum xam tus naj npawb ntawm cov ntsiab lus tsawg kawg yog ib puas loj squares. Cov txiaj ntsig tau muab faib los ntawm 1600, tom qab ntawd nws tau muab faib ua 4000, thiab tom qab ntawd los ntawm 20 (lub degree ntawm dilution).
